This report aims to demonstrate that common laboratory materials and a simple protocol can be used to standardize the preparation of PRP. The emphasis is on preserving platelets' physiological properties. This protocol can be replicated in any clinical setting. It allows for cost-effective delivery and management of growth factors that may be useful in managing burns.
The hospital pharmacy prepared the kits for blood harvest. These kits include 4.5 mL Vacutainer tubes containing sodium citrate solution. The final concentration is 3.8% sodium citrate (0.129M). A butterfly valve can also be attached to a 20ml syringe. It has Cambi caps that seal the end of the syringe after blood harvesting. Also, an adapter measuring 19mm is available. Those in need can buy EmCyte PRP kits for sale from #1 PRP Kit Suppliers in New Jersey for appropriate PRP preparation.
Method for preparation of PRP from blood and centripetal
You can draw whole blood directly from the patient's vein using four 4.5mL citrates (3.8% salt citrate)-containing a Vacutainer tube (clinical grade, borosilicate, glass of non-pyrogenic and non-cytotoxic, nuclease-free) or a syringe to extract 3.8% citrate solution sterile from a pharmaceutical preparation. Then attach the syringe to the butterfly valve before drawing blood.
This is done in the outpatient clinic, which is located next to the hospital's preparation laboratory. It allows for quick transfer and preparation. The blood is gently transferred to a centrifuge tube of 50 ml size under a laminar flow cover. The Megafuge centrifuge is used to centrifuge the PRP at 280g for 15 minutes at room temperature.
After centrifugation, remove the PRP with a pipette. This is inserted over the buffy layer and transferred to a new, sterilized centrifuge tube. The second centrifugation is done under the same conditions. The concentrated PRP is then taken with a sterilized syringe to be used in clinical applications.